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C38 - Creating a Foxe3 null lens epithelial cell line via CRISPR Cas9 gene editing
The use of cultured cell lines can sometimes provide a cost-effective alternative to transgenic animal models for studying gene function and signaling pathways.
C38 - Creating a Foxe3 null lens epithelial cell line via CRISPR Cas9 gene editing
Mentor(s): Mike Robinson, Ph.D., Brad Wagner, Ph.D.
The use of cultured cell lines can sometimes provide a cost-effective alternative to transgenic animal models for studying gene function and signaling pathways.1 However, validation that a given cell line resembles its primary cell type is critical to confidently draw credible conclusions of any given study. The goal of this project is to create a transgenic clonal population of mouse lens epithelial cells lacking a functional Foxe3 gene. CRISPR/Cas9 gene editing technology will be used to induce a frame-shift mutation in the Foxe3 gene in the 21EM15 lens epithelial cell line. RNA sequencing will be used to compare the Foxe3 null cell line to wild-type 21EM15 cells to gain insights into the genes controlled by FOXE3. This RNA-seq data will also be used to assess congruency between Foxe3 null and wild type 21EM15 cells and existing RNA-seq data from wild-type and Foxe3 null mice.